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Fig. 4 | <t>UPF1</t> is necessary for Igh VH-DHJH recombination at early LPre-B stage. a–d The transcript amount of Ighv (a), Ighm (b), Ighj (c), and Ighd (d) in Upf1-cKO and Ctrl early LPre-B cells. The data were obtained from the RNA-seq shown in Fig. 2 (n = 3). Each bar represents the mean ± SD from biological replicates. e Schematic illustration of germline Igh locus. Colored arrows indicate each primer used in PCR analysis in (f). f PCR assay amplifying recombined Igh as shown in (e) using the DNA derived from Upf1-cKO and Ctrl early LPre-B cells. Genome DNA derived from the tail of the WT mouse was used as a negative control. Results are representative of at
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Fig. 4 | <t>UPF1</t> is necessary for Igh VH-DHJH recombination at early LPre-B stage. a–d The transcript amount of Ighv (a), Ighm (b), Ighj (c), and Ighd (d) in Upf1-cKO and Ctrl early LPre-B cells. The data were obtained from the RNA-seq shown in Fig. 2 (n = 3). Each bar represents the mean ± SD from biological replicates. e Schematic illustration of germline Igh locus. Colored arrows indicate each primer used in PCR analysis in (f). f PCR assay amplifying recombined Igh as shown in (e) using the DNA derived from Upf1-cKO and Ctrl early LPre-B cells. Genome DNA derived from the tail of the WT mouse was used as a negative control. Results are representative of at
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Fig. 4 | <t>UPF1</t> is necessary for Igh VH-DHJH recombination at early LPre-B stage. a–d The transcript amount of Ighv (a), Ighm (b), Ighj (c), and Ighd (d) in Upf1-cKO and Ctrl early LPre-B cells. The data were obtained from the RNA-seq shown in Fig. 2 (n = 3). Each bar represents the mean ± SD from biological replicates. e Schematic illustration of germline Igh locus. Colored arrows indicate each primer used in PCR analysis in (f). f PCR assay amplifying recombined Igh as shown in (e) using the DNA derived from Upf1-cKO and Ctrl early LPre-B cells. Genome DNA derived from the tail of the WT mouse was used as a negative control. Results are representative of at
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Fig. 4 | <t>UPF1</t> is necessary for Igh VH-DHJH recombination at early LPre-B stage. a–d The transcript amount of Ighv (a), Ighm (b), Ighj (c), and Ighd (d) in Upf1-cKO and Ctrl early LPre-B cells. The data were obtained from the RNA-seq shown in Fig. 2 (n = 3). Each bar represents the mean ± SD from biological replicates. e Schematic illustration of germline Igh locus. Colored arrows indicate each primer used in PCR analysis in (f). f PCR assay amplifying recombined Igh as shown in (e) using the DNA derived from Upf1-cKO and Ctrl early LPre-B cells. Genome DNA derived from the tail of the WT mouse was used as a negative control. Results are representative of at
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Fig. 4 | UPF1 is necessary for Igh VH-DHJH recombination at early LPre-B stage. a–d The transcript amount of Ighv (a), Ighm (b), Ighj (c), and Ighd (d) in Upf1-cKO and Ctrl early LPre-B cells. The data were obtained from the RNA-seq shown in Fig. 2 (n = 3). Each bar represents the mean ± SD from biological replicates. e Schematic illustration of germline Igh locus. Colored arrows indicate each primer used in PCR analysis in (f). f PCR assay amplifying recombined Igh as shown in (e) using the DNA derived from Upf1-cKO and Ctrl early LPre-B cells. Genome DNA derived from the tail of the WT mouse was used as a negative control. Results are representative of at

Journal: Nature communications

Article Title: UPF1 plays critical roles in early B cell development.

doi: 10.1038/s41467-024-50032-6

Figure Lengend Snippet: Fig. 4 | UPF1 is necessary for Igh VH-DHJH recombination at early LPre-B stage. a–d The transcript amount of Ighv (a), Ighm (b), Ighj (c), and Ighd (d) in Upf1-cKO and Ctrl early LPre-B cells. The data were obtained from the RNA-seq shown in Fig. 2 (n = 3). Each bar represents the mean ± SD from biological replicates. e Schematic illustration of germline Igh locus. Colored arrows indicate each primer used in PCR analysis in (f). f PCR assay amplifying recombined Igh as shown in (e) using the DNA derived from Upf1-cKO and Ctrl early LPre-B cells. Genome DNA derived from the tail of the WT mouse was used as a negative control. Results are representative of at

Article Snippet: The following primary antibodies were used for immunoblot analysis: antibody to UPF1 (NBP1-05967, Novus biologicals), Phospho-UPF1(071016, Merck), β-actin (sc-1615, Santa Cruz) The complete list of antibodies used in this study can be found in Supplementary table 2.

Techniques: RNA Sequencing, Derivative Assay, Negative Control